Oral Presentation Australian & New Zealand Obesity Society 2014 Annual Scientific Meeting

Influence of carotenoids on oxidative damage, inflammation and [NAD(H)] in human cerebrospinal fluid with age (#41)

Ross S Grant 1 , Jade Guest 1 , Trevor Mori 2 , Manohar Garg 3 , Kevin Kroft 2 , Ayse Bilgin 4
  1. Sydney Adventist Hospital, Wahroonga, NSW, Australia
  2. School of Medicine and Pharmacology, University of Western Australia, Perth, WA, Australia
  3. School of Biomedical Sciences and Pharmacy, University of Newcastle, Newcastle, NSW, Australia
  4. Faculty of Science, Macquarie University, Sydney, NSW, Australia

Evidence indicates that oxidative stress and inflammation play a central role in the degenerative changes of systemic tissues in aging. However a comparatively limited amount of data is available to verify whether these processes also contribute to normal aging within the brain. In this study we quantified changes in [NAD(H)] and markers of inflammation and oxidative damage (F2-isoprostanes, 8-OHdG, total antioxidant capacity) in the cerebrospinal fluid (CSF) of healthy humans across a wide age range (24-91 years). The effect of plasma carotenoid concentrations, a group of dietary derived phytochemicals with potent antioxidant and anti-inflammatory properties, was also evaluated. CSF of participants aged >45 years contained increased levels of lipid peroxidation (F2-isoprostanes) (p=0.04) and inflammation (IL-6) (p=0.00) and decreased levels of both total antioxidant capacity (p=0.00) and NAD(H) (p=0.05), compared to their younger counterparts. After adjusting for age and gender, total antioxidant capacity correlated positively with both -carotene (p=0.01) and -carotene (p<0.001) in plasma. An inverse correlation was seen between plasma lycopene and the plasma inflammatory cytokine IL-6 (p=0.02). An increase in plasma -cryptoxanthin correlated with a decrease in CSF IL-6 (p=0.04). A significant positive correlation was found between plasma lycopene and both plasma (p<0.001) and CSF (p<0.01) [NAD(H)]. Surprisingly no statistically significant associations were found between the most abundant carotenoids, lutein + zeaxanthin and markers of oxidative stress in the plasma or CSF. These data suggest a progressive age associated increase in oxidative damage, inflammation and reduced [NAD(H)] in the brain which may be moderated by the consumption of specific dietary carotenoids.